富氢水(氢气)的抗皱美容效果研究
小春日语-竞聘上岗演讲稿
富氢水（氢气）的抗皱美容效果研究
系统分类:科研笔记|关键词:抗皱 美容
style class
富氢水（氢水）洗澡3个月可以显著减少皮肤皱纹。
Hydrogen-rich electrolyzed warm water
represses wrinkle formation against UVA
不久前曾经
有韩国学者用动物试验证明《氢气洗澡能对抗紫外
线》引起的动物皮肤损伤。本研究采用体外细胞学研究
并进行了人体
试验。使用温电解富氢气水，温度为41度，氢气的溶解量为1.13
ppm，
氧化还原电位为-741 mV。普通水中氢气的含量为0.01
ppm以下（没
有测定出），氧化还原电位为+184 mV。选择皮肤相关的纤维母细胞
OU
MS-36和角质细胞HaCaT用于紫外线损伤试验。结果发现，3-5
天后氢水可以使OUMS-3
6细胞I型胶原合成增加1.85到2.03倍，
免疫组织化学染色显示I型胶原主要分布在细胞核周围
。细胞增殖及
细胞毒性（WST-1）和细胞凋亡与坏死（Hoechst 33342
）检测结果
发现，氢气水可以显著保护HaCaT角质细胞的DNA损伤，如细胞
核固缩、核酸
片断形成等。氯化硝基四氮唑蓝（NBT）光化还原法证
明氢气对两种细胞的超氧阴离子产生都具有抑制
作用。
人体抗皱效应试验采用氢气水沐浴，6名日本人参加，每天使
用氢气含量为0.2-0.4 p
pm的水进行沐浴，连续3个月。通过第90
天与试验前颈和背部皮肤的比较，发现其中4名受试者颈和
背部皮肤
皱纹明显减少。研究提示，氢气水洗可通过清除活性氧作为日常皮肤
防紫外线辐射损伤
和抗皱手段。
这是来自日本最新研究，文章发表在Journal of
Photochemistry
and Photobiology B:
Biology。题目为Hydrogen-rich electrolyzed warm
water represses wrinkle formation against UVA
ray together with
type-I collagen production
and oxidative-stress diminishment in
fibroblasts and cell-injury prevention in
keratinocytes
来自日本广岛大学，生命与环境科学系的Shinya
Kato
a, 1
,
Yasukazu Saitoh
a, 2
,
Keizou Iwai
b
, Nobuhiko Miwa等作者认为，
本研究的亮
点为：通过试验证明，氢气水可促进纤维细胞胶原合成，
清除自由基，抑制角质细胞死亡。临床研究证明
氢气水沐浴具有抗皱
效应。
本人评论，虽然这个研究非常有意思，毕竟是关于抗皮肤
衰老
首次进行的人体试验。但研究存在一些缺陷，细胞学试验证据不足，
没有针
对胶原蛋白合成的原因进行探讨，实际上皮细胞合成胶原的调
节方式已经比较清楚，氢气如何影响这些途
径是非常容易进行分析和
研究的，至少要针对一些比较重要的信号分子进行检测。人体试验样
本
量太少，只有6个，而且只有4个有效，必须有10-20个人的数
量才能算小样本的小型试验，这样的
小规模试验无法证明这种手段的
有效性。很难排除偶然性和人为因素的干扰。
英文摘要和目录
Hydrogen-rich electrolyzed
warm water (HW) was prepared at 41°C and
exhibited dissolved hydrogen
(DH) of 1.13
ppm and an oxidation-reduction potential (ORP) of
-741 mV in contrast to below 0.01 ppm and +184
mV for regular warm water (RW). Fibroblasts
OUMS-36
and keratinocytes HaCaT were used to
examine effects of HW against UVA-ray irradiation.
Type-I
collagen was synthesized 1.85- to
2.03-fold more abundantly by HW application for
3-5 days than RW in
OUMS-36 fibroblasts, and
localized preferentially around the nuclei as
shown by immunostain. HW
application
significantly prevented cell death and DNA damages
such as nuclear condensation and
fragmentation
in UVA-induced HaCaT keratinocytes as estimated by
WST-1 and Hoechst33342 assays.
HW
significantly suppressed UVA-induced generation of
intracellular superoxide anion radicals in both
the cell lines according to NBT assay. Wrinkle
repression was clinically assessed using a HW-
bathing.
Six Japanese subjects were enrolled
in a trial of HW-bathing (DH, 0.2-0.4 ppm) every
day for 3 months.
HW-bathing significantly
improved wrinkle in four subjects on the back of
neck on 90 day as compared
to 0 day. Thus, HW
may serve as daily skin care to repress UVA-
induced skin damages by
ROS-scavenging and
promotion of type-I collagen synthesis in
dermis.
th
Highlights
►
Hydrogen-rich electrolyzed warm water (HW)
promotes the type-I collagen synthesis in
fibroblasts and
suppresses cell death in
keratinocytes, in accompanying with quenching UVA-
derived intracellular
reactive oxygen species.
► In addition, a clinical study of HW-bathing
demonstrated the
repressive-wrinkle formation
on 90 day as compared to 0 day. ► Therefore,
continuous application of
HW reduces oxidative
stress in the skin, and expects to prevent the
wrinkle formation.
(
'embedAPCModule');Keywords:Hydrogen
water; UV-A; Collagen type-I; Fluorescent
immunostaining;
Oxidative stress; Hydrogen
water bathing; Wrinkle repression
th
Article Outline
1.
Introduction
2. Materials and methods
2.1. Cell culture
2.2.
Preparation of Hydrogen-rich Electrolyzed Warm
Water (HW) and HW-containing medium
2.3. Application of Hydrogen-rich
electrolyzed warm water (HW) and Immunostaining
for type I collagen
in OUMS-36 fibroblasts
2.4. Intracellular ROS-generation examined by
NBT assay in OUMS-36 fibroblasts
2.5. Cell
viability measured by WST-1 assay in relation to
DNA -cleavage and intracellular ROS
generation
in HaCaT keratinocytes
2.6. Clinical
evaluation of repressive-wrinkle formation by
daily HW-bathing
2.7. Statistical
analysis
3. Results
3.1.
Effects of HW on type-I collagen production and
antioxidant activity against UVA in OUMS-36
fibroblasts
3.2. Defensive effects of
HW against UVA-induced injury in HaCaT
keratinocytes
3.3. Clinical evaluation of
repressive-wrinkle formation by HW-bathing
4. Discussion
5. Conclusions
References
The device for HW-
bathing and dissolved hydrogen level (ppm) of
bathwater in supplying
horse and bath-tub.
This device can supply HW by electrolysis of tap-
water and hold
dissolved hydrogen at the lev
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